Journal: iScience

Article Title: Cdk6’s functions are critically regulated by its unique C-terminus

doi: 10.1016/j.isci.2024.111697

Figure Lengend Snippet: Model (A) Graphical representation of our model displaying the consequences of a shorter C-terminal domain on Cdk6 functions. The shorter C-terminal domain of Cdk6 ΔC reduces its protein flexibility. This in turn causes the loss of Cdk6 ΔC interactions with partners crucial for cell cycle progression (cyclin D) and for its nuclear translocation (p27 Kip1 and p16 INK4a ), therefore perturbing its chromatin interactions. The binding of cyclin B is not affected by the C-terminus of Cdk6.

Article Snippet: Rabbit polyclonal anti-p27 Kip1 , Proteintech , Cat# 26714-1-AP; RRID: AB_2880611.

Techniques: Translocation Assay, Binding Assay

Journal: iScience

Article Title: Cdk6’s functions are critically regulated by its unique C-terminus

doi: 10.1016/j.isci.2024.111697

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-p27 Kip1 , Proteintech , Cat# 26714-1-AP; RRID: AB_2880611.

Techniques: Recombinant, Luciferase, Magnetic Beads, Immunoprecipitation, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Modification, cDNA Synthesis, SYBR Green Assay, Western Blot, Bradford Protein Assay, Software

Journal: The Indonesian Biomedical Journal

Article Title: Stenochlaena palustris Ethanol Extract Decreases Viability and Induces G1-Phase Cell Cycle Arrest in HSC-3 Tongue Cancer Cells via p21 and p27

doi: 10.18585/inabj.v16i5.3308

Figure Lengend Snippet: Figure 4. SPEE increased p27 amount of HSC-3 cells. HSC- 3 cells were treated with/without 1 µM Doxorubicin or SPEE in various concentrations. The p27 amount was assessed with Immunoblotting assay, as described in the Methods. *significant (Tukey’s post hoc test, p<0.05) compared with Sham group.

Article Snippet: For p27 Kip1 detection, the previously quantified membrane for p21 Waf1/Cip1, was stripped, washed, re-blocked, re-probed with rabbit polyclonal anti-p27 Kip1 primary antibody (Cat. No. 2552; Cell Signaling) and HRP-conjugated goat antirabbit secondary antibody, visualized with Clarity Western ECL, captured, analyzed and quantified with Alliance 4.7.

Techniques: Western Blot